DNA

Part:BBa_K4577004:Design

Designed by: Ma Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-03)


Hao


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1213
    Illegal XhoI site found at 1384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 43
    Illegal BsaI site found at 273
    Illegal BsaI.rc site found at 142
    Illegal BsaI.rc site found at 709
    Illegal SapI site found at 1350


Design Notes

This part is optimized for the bacterial strain Sinorhizobium meliloti. So, the main consideration is the codon preference of the strain


Source

It is a plasmid sequence

References

[1] Fan Ouyanga, Dan Penga,Xiao Su, Population dynamics and metabolic alternations of AOB in activated sludge under Cu shock loads, Desalination and Water Treatment, october 2020, doi: 10.5004/dwt.2020.26102